Dr. Lucio Costa and co-workers describe six different behavioral tests in the three main areas affected in ASD (repetitive behavior, communication, social interactions).
Umbilical Cord Mesenchymal Stromal Cell Isolation and Characterization
New protocols for the isolation of UC-MSCs are explored, including several helpful, individual protocols to aid quality control and ensure that the UC-MSCs are characterized thoroughly while improving yield.
Combining Fluorescence and Electron Microscopy for 3D Cell Imaging
Combining the high-throughput and large scale analysis of fluorescence microscopy with the enhanced resolution capabilities of electron microscopy can allow imaging of cellular interactions and mechanisms without being limited by resolution.
How Referencing Can Impact Reproducibility
Did you know that your citing habits can influence the reproducibility of your work?
Research Done Right: 3 Tenets of a Successful Research Approach
Research may well be a creative, fluid and dynamic field, but structure and high standards are required for success.
Functional microRNA Screen for Organ Morphogenesis
A detailed protocol to assess microRNA function in explanted organs is presented.
A High-Throughput Cell Chemotaxis and Migration Inhibitor Screen
A protocol for a unique high-throughput assay to identify novel small-molecule inhibitors of cell migration.
Making Insulin Producing Beta Cells by Cell Reprogramming
A robust and reproducible method for “direct programming” of acinar cells into functional beta cells with a polycistronic viral construct and viral transduction of pancreatic tissue in vivo in mice is described.
Rapid, Low Cost Detection of E.coli and Shigella
A 16S rRNA targeting oligonucleotide probe to improve target organism specificity and lowers costs for detection of low-level E. coli/Shigella contamination.
Magnetic Nanoparticle-Mediated Gene Delivery to Neural Stem Cells
The first description on the use of oscillating magnetic fields for magnetic nanoparticle-mediated (MNP) gene transfer to neural stem cells NSCs.