“Why are we interested in monocytes?”
Claude Lambert and Ulrich Sack, guest editors of Special Issue on Monocytes and Macrophages , ask the Cytometry Part B: Clinical Cytometry readers this question at the beginning of their Introduction to this special issue.
Monocytes are large white blood phagocytic cells with no distinct features, no immune specificity, no memory, and no sizable representation in peripheral blood. And their destiny is uncertain. Often they are ignored, disregarded, and even considered as a contaminant in blood samples prepared for the analysis of mature lymphocytes or granulocyte populations.
But don’t judge a book by its cover.
Besides maturing into macrophages and myeloid lineage dendritic cells, monocytes are indispensable in early differentiation in bone marrow and late maturation stages in tissue, between innate and adaptive immune responses, between gross phagocytosis and fine antigen presentation, and even in modulating cytokine release.
3 editorials, 1 review and 4 articles below present different views on the same cell, about its development, physiology, signaling, and production of reactive species, fitness, exhaustion, dysplasia, and leukemia that can be interrogated by flow cytometry:
Monocytes and macrophages in flow: an ESCCA initiative on advanced analyses of monocyte lineage using flow cytometry
Claude Lambert, Frank W. M. B. Preijers, Gulderen Yanikkaya Demirel and Ulrich Sack
CD64 expression by neutrophil granulocytes
CD163 and CD206 expression does not correlate with tolerance and cytokine production in LPS-tolerant human monocytes
Amanda Barba Alves-Januzzi, Milena Karina Colo Brunialti and Reinaldo Salomao
Flow cytometry in the diagnosis of myelodysplastic syndromes and the value of myeloid nuclear differentiation antigen
Frauke Bellos and Wolfgang Kern
Flow cytometric analysis of intracellular phosphoproteins in human monocytes
Emilie Coppin, Fabrice Malergue, Marie-Laure Thibult, Caroline Scifo, Cédric Favre and Jacques A. Nunès
Real-time cytometric assay of nitric oxide and superoxide interaction in peripheral blood monocytes: A no-wash, no-lyse kinetic method
Susana Balaguer, Laura Diaz, Angela Gomes, Guadalupe Herrera, José-Enrique O’Connor, Amparo Urios, Vicente Felipo and Carmina Montoliu
Introduction to the diagnosis and classification of monocytic-lineage leukemias by flow cytometry
Sergio Matarraz, Julia Almeida, Juan Flores-Montero, Quentin Lécrevisse, Valentina Guerri, Antonio López, Susana Bárrena, Vincent H. J. Van Der Velden, Jeroen G. Te Marvelde, Jacques J. M. Van Dongen and Alberto Orfao
Aggregation of monocytes and platelets interferes in measurement of monocyte viability with phosphatidylserine expression but not with Mitochondrial membrane potential in whole blood
Erik K. Amundsen, Petter Urdal, Mette R. Holthe and Carola E. Henriksson