A protocol for generating retinal organoids from human pluripotent stem cells that contain all major retinal cell types.
Current Protocols Authors
Current Protocols is a collection that includes nearly 20,000 step-by-step techniques, procedures, and practical overviews that provide researchers with reliable, efficient methods to ensure reproducible results and pave the way for critical scientific discovery. With its emphasis on carefully curated, highly edited methods rich in detail, practical advice, and troubleshooting, Current Protocols enables researchers to advance their research with an efficiency of time and resources.
Generation of Pancreatic Ductal Organoids
Intact whole-mount immunostaining of organoids after growth for 14 days.
Scalable Method to Generate Human iPSC-Derived Macrophages
Protocol for iPSC-derived macrophages with excellent functional and transcriptomic fidelity.
Organotypic Culture System for in vitro Bone Modeling
An organotypic culture system that allows the production of bone tissue features on a centimeter scale.
Systematic Mass Spectrometry‐based Method for Spatial Proteomics
Proteome-wide analysis of protein subcellular localization changes.
Live-Imaging of Human Brain Organoids
Low cost, on-chip, high-resolution imaging to study cellular dymamics of intact brain organoids.
Expansion Microscopy Enabling Nanoscale-Resolution Imaging of Preserved Cells and Tissues
Step‐by‐step Expansion Microscopy protocols for performing analysis of proteins and RNAs using chemicals and hardware found in a typical biology lab.