The new Raman microscope system includes laser wavelength selection with subsequent adjustment of all associated spectrometer and microscope components to ensure optimized system performance. The absolute laser power determination and regulation in 0.1 milliwatt steps preserves delicate samples and guarantees the reproducibility of measurement conditions. The “UHTS 600”, a new 600 mm focal length spectrometer designed specifically for automated Raman imaging, enables challenging experiments at even very low light intensities. Versions are available for a wide variety of excitation wavelengths and optimized for their specific lasers.
Spectral resolution of the “apyron” system is unprecedented, down to 0.1 rel 1/cm per pixel (@633 nm excitation). Highly detailed spectral information is given for every image pixel, and more than 16 mio. Raman spectra can be acquired in a single dataset. The system setup includes TrueSignal to maximize the light into the fiber output of the microscope. Thus, the system delivers images with outstanding spectral and spatial resolution in 3D, limited laterally only by physical law. While downright sophisticated, it is also an easy-to-use, push-button instrument. Pre-defined calibration routines are integrated to facilitate quick and user-friendly system maintenance with consistently high system performance.