Combining of Fluorescence Microscopy with an AFM

by | Jun 28, 2016

Combination of Atomic Force Microscopy (AFM) with the single-molecule-sensitive fluorescence microscopy platform “MicroTime 200” that grants fascinating insights into the structure, dynamics, and interactions of single molecules or their assemblies in cells.
PicoQuant - MicroTime 200

PicoQuant – MicroTime 200

The time-resolved microscopy platform can be interfaced to a series of AFMs from Bruker, JPK, and Asylum Research while maintaining its full range of capabilities such as super-resolution via STimulated Emission Depletion (STED). The AFM part reveals structural information of macromolecular complexes on the nanometer scale, while fluorescence lifetime data facilitates the identification of their constituent parts. The integration of both techniques in a single instrument opens up new perspectives for live cell imaging. Thanks to seamless communication and state-of-the-art synchronization between the AFM and time-resolved fluorescence microscope, you will obtain superior AFM data without sacrificing optical image quality.

Potential applications include live cell imaging, where the effect of protein changes on cell shape and structure can be investigated, merging of sub-nanometer AFM topographic imaging with optical encoded functionality or investigating inter- and intramolecular distances using force spectroscopy.

PicoQuant GmbH
Rudower Chaussee 29
12489 Berlin, Germany
Phone: +49 (0)30 6392-6929
Fax: +49 (0)30 6392-6561