Supply: New method “rapidFLIM” for dynamic Fluorescence Lifetime Imaging (FLIM) that significantly accelerates data acquisition.
Background: FLIM is a very versatile microscopy method combining fluorescence lifetime information with spatial localization in the sample. This method allows investigating, for example, biochemical and physical processes, detecting changes in the local environment of the sample, molecular interactions, or conformational changes via Förster resonance energy transfer (FRET). FLIM data acquisition is typically based on time-correlated single photon counting (TCSPC) electronics. Up to now, CSPC data acquisition has been considered a somewhat slow process, due to the time required to collect a sufficient number of photons per pixel for reliable data analysis.
Features: The novel “rapidFLIM” method allows acquiring several FLIM images per second by exploiting recent hardware developments in TCSPC modules with ultra-short dead times and hybrid photomultiplier detector assemblies. Depending on image size and sample brightness, more than 15 images can be acquired per second, making it possible to follow dynamic processes such as transient protein interaction in living cells or chemical reactions as well as highly mobile species without sacrificing the lifetime contrast or the high spatial resolution of the confocal microscope.